Accurate and Sensitive Detection of Plasmodium Species in Humans by Use of the Dihydrofolate Reductase-Thymidylate Synthase Linker Region
Tanomsing N., Imwong M., Theppabutr S., Pukrittayakamee S., Day NPJ., White NJ., Snounou G.
ABSTRACT A nested-PCR protocol based on the linker region of the Plasmodium dihydrofolate reductase-thymidylate synthase gene ( dhfr-ts ) was developed. This provides highly sensitive specific detection and identification of the five parasite species that infect humans.