Genetic Marker Suitable for Identification and Genotyping of Plasmodium ovale curtisi and Plasmodium ovale wallikeri
Tanomsing N., Imwong M., Sutherland CJ., Dolecek C., Hien TT., Nosten F., Day NPJ., White NJ., Snounou G.
ABSTRACT We present a seminested PCR method that specifically discriminates between Plasmodium ovale curtisi and P. ovale wallikeri with high sensitivity. The test is based on species-specific amplification of a size-polymorphic fragment of the tryptophan-rich antigen gene, potra , which also permits discrimination of intraspecific sequence variants at this locus.